WHAT IS IN-VITRO FERTILIZATION TREATMENT?
In-vitro fertilization treatment is based on fertilization of the oocytes taken from ovaries and the sperms taken from male partner outside of the body and transferring the obtained embryos to the uterus of the future mother. In-vitro fertilization is a treatment method applied to women who are not conceived by classical methods. It is based on the principle of transferring the embryos which are formed by combining male (sperm) and female (oocyte) cells in laboratory conditions to the uterus. Fertilization provided in laboratory conditions is achieved by transferring a sperm into an egg either spontaneously or by human hand.
What Are In-vitro Fertilization Methods?
What Are the Steps of In-Vitro Fertilization Treatment?
Frequently Asked Questions
The medical history of couples, age of the woman, quality of the sperm, quality of the embryo, the capacity of uterus to hold the embryo affect the success.
There is no need for hospitalization at any stage of in-vitro fertilization treatment.
The protocol is determined by considering examination findings, ovarian reserve, the age of the person, previous protocols and sperm parameters of the male partner.
There is no any differences between treatment pregnancy and spontaneous pregnancy.
There is no differences between method of delivery in-vitro fertilization pregnancy and spontaneous pregnancy.
The process starts with the detection of tests and intervention in which couples need to be evaluated together according to the planned treatment. And continue ovulation induction process, oocyte pick-up under general anesthesia, and transfer of embryos into the uterus.. Whole treatment is completed approximately in 20 days.
There is no upper limit to the treatment. It can be tried as long as the depends on ovarian reserve. . As the number of the attempts -increases, the chance of pregnancy also increases. However, depends on advanced maternal age the pregnancy rate unfortunately decrease
-Diagnosis and treatment plan are the most important factors affecting the pregnancy rate -. In addition, medications and methods used to support the treatment; such as vitamin supports, pre-genetic diagnosis (PGD), assisted hatching, paternal lymphocyte immunization ,laparoscopy, hysteroscopy, laboratory techniques, quality incubators, transfer catheters; are the factors increasing the success rate in IVF treatment.
The duration of the OPU process is related to the number of follicles in the ovaries. But it can takes 15-30 minutes on average.
One of the most important factors for the success of IVF treatment is the maternal age that decreases according to the age of woman.. Also the number and quality of oocytes are lower than the young patients. Women’s fertility decreases after the age of 35 and this also accelerates after the age of 38. For the women older than 40, the chance of pregnancy with in vitro fertility treatment falls below 20%. In addition, the risk of miscarriage at these ages is high. For women who are older than 38 years, the condition of the embryos needs to be evaluated with preimplantation genetic diagnosis (PGD) technique during in vitro fertilization. For 15 days before the start of the IVF treatment in older women medication treatment is started for the stimulation of the eggs.
Physical examination is one of the most important steps of infertility evaluation. Gynecologic and ultrasonographic examinations of the female partner, pathologic examination by smear, microbiological examinations and hormone tests (FSH, LH, E2, PRL, TSH, T3, T4 on the 3rd day of menstruation); are required. Semen analysis (spermiogram) test should be done for male partner. If sperm count is seriously low or there is no sperm, hormonal tests and genetic examination should be done – If necessary, further diagnostic methods such as uterus x-ray, hysteroscopy and laparoscopy can be used.
– TESE method performed under general anesthesia, sperm cells are searched by examining a large number of parts taken from different regions of testicles. Achieved sperm cells are separated and prepared for microinjection. With the advancements in technology field, the Micro-TESE method has emerged. The Micro-TESE method is performed under the microscope compared to the Classic TESE method, and in this case tissues are more likely to contain sperm.
The chance of finding sperm cells in Azoospermia patients is about 40% higher with the Micro-TESE method compared to the Classic TESE -. In addition, the Micro-TESE method causes less damage to testicular tissue. Sperm cells can be achieved by the Micro-TESE method up to 7% in patients who have not had sperm cells -aciheved before by the classical TESE method. – The probability of finding sperm cells by Micro-TESE method is up to 35% in patients without obstruction complaint.
Both methods are performed under general anesthesia but in some cases local anesthesia is also sufficient for the Micro-TESE procedure. Since the micro-TESE procedure is performed under the microscope, the probability of damaging the vessels in the testicles is lower than the TESE procedure.- In both procedures, the testicle is examined in detail and each section that is likely to have sperm is searched one by one. Both procedures takes 1 hour. –
With the microinjection method, the sperm is placed inside the oocyte under the microscope, but this does not mean that fertilization will occur in every application. The quality of sperm and quality of -oocyte are also important in this method. Fertilization and pregnancy rate are higher in microinjection method than in the traditional IVF method. However; mother’s age is also effective in this method. Depending on the age and health condition, the chances of achieving fertilization with microinjection changes between 50% and 80%, but there is no guarantee that all of these fertilizations will result in pregnancy. –